Table 2 Primers used for quantitative RT-PCR (qPCR)
Target genes | Sources | primer (5′-3′) |
|---|---|---|
GAPDHa | Y09455.1 | F: ACTGTCACTCCTCCATCTT |
R: CACGGTTGCTGTATCCAA | ||
EF-1αb | AF467776.1 | F: TCATTGGCCATGTCGACTCC |
R: ACGTAGTACTTGGCGGTCTC | ||
TORc | JN850959.1 | F: TCTCCCTGTCCAGAGGCAATAA |
R: CAGTCAGCGGGTAGATCAAAGC | ||
AKTd | MG516906.1 | F: AGCGCACCTTCCATGTAGAC |
R: GGCTATTTGCCACTTGCTGG | ||
S6e | XP_010747297.3 | F: GTAATGCAAAGGACACGGCG |
R: GTTCCCCACCGCTCAGATAC | ||
4EBP1f | NM_004095.4 | F: AGCAGGAACTTTCGGTCATA |
R: GTCAATGGGCAGTCAGAAGA | ||
GHg | KR269816.1 | F: GACGGAGGAGCAGCGACA |
R: ACAGGGACCGACTGGGGA | ||
IGF-1h | AY776159.1 | F: TATTTCAGTAAACCAACAGGCTATG |
R: TGAATGACTATGTCCAGGTAAAGG |