Fig. 7
Effect of FSH, E2 and its receptor agonists on dissociated ovarian cell in vitro. A: Left panel showed the microscopy observation of dissociated ovarian sup-population cells including the germ cells (○) characterized by conspicuous size, large round nuclei (Nu), and spherical balbiani body (Ba); steroidgenic cells (□) showing large amount lipid droplet, and Somatic cells (□) of irregular shape. Middle panel indicated the results of immunohistochemical localization steroid hormone synthesis pathway key enzyme 3β-HSD and germline marker DDX4 for the dissociated ovarian cell sub-population identification. Right panel indicated the results of trypan blue exclusion assay (the excluded and penetrated cells were marker by solid and dotted boxes respectively ). B and C. Fluorescence images of the TUNEL staining and quantification of the TUNEL-positive labbled dissociated ovarian cell under FSH (0.5 IU/mL), E2 (10− 3 mM/mL), co-treatment with FSH(0.5 IU/mL) and E2(10− 3 mM/mL), and DPN dose-treatment (0.025 mM/ml, 0.1 mM/ml, and 0.25 mM/ml); D. The mRNA expression of pre-meiotic marker Scyp3. The significant differences are indicated with different letters (P < 0.05), the same letter means no significant difference (P > 0.05)