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Fig. 12 | BMC Zoology

Fig. 12

From: Microanatomy of the female reproductive system of the viviparous freshwater whipray Fluvitrygon signifer (Chondrichthyes: Myliobatiformes: Dasyatidae). II. The genital duct

Fig. 12

Histochemistry of the vaginal epithelium of F3, F4 and F5 females of Fluvitrygon signifer. The vaginal epithelium of F5 female (a, b) showing a desquamation border of the epithelium indicated by black triangles (b). Determination of proteins (c) and metachromatic property (d) of the granules. e, f Posterior region of the vagina having epithelial cell types. Specimen codes indicate their corresponding micrographs. Abbreviations: BC, basal cell; DC: desquamated cell; Gr, granule; Gr1–3, type 1–3 granules with different levels of metachromasia; Lc, leukocyte; MC, mucous cell; NC, non-secretory cell. Stains: a, e = PAS-H; b, f = modified Movat’s pentachrome; c = bromophenol blue; d = toluidine blue

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