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Fig. 5 | BMC Zoology

Fig. 5

From: The neotypification of Frontonia vernalis (Ehrenberg, 1833) Ehrenberg, 1838 and the description of Frontonia paravernalis sp. nov. trigger a critical revision of frontoniid systematics

Fig. 5

TEM (a-c) and FISH images (d, e), and conjugation process after Feulgen staining (f-j) of Frontonia paravernalis sp. nov. a Detail of macronucleus (Ma), micronucleus (Mi), Chlorella-like endosymbiont (Ch), and unknown particle (arrowhead); b closer view of Chlorella-like endosymbiont (Ch); c detail of unknown particles (arrowhead); d, e FISH images showing autofluorescent Chlorella-like endosymbionts (Ch) and unknown particles (double arrowhead) negative to eubacterial (d) and alphaproteobacterial (e) probes; f conjugant pair; g exconjugant with still not macronucleus (Ma); h exconjugant with new macronuclear anlagen (MaA) and old macronuclear fragments (MaF); i closer view of MaF and micronuclei (Mi); j detail of new MaA, MaF, and Mi. Ch – Chlorella-like endosymbiont; Ma – macronucleus; MaA – macronuclear anlagen; MaF – macronuclear fragments; Mi – micronucleus; Nu – nucleus; PL – plastid; Arrowhead – unknown particle. Bars stand for 25 μm (f-h), 10 μm (d, e), 5 μm (i, j), 1 μm (a-c)

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