Fig. 4

Effect of nocturnal light irradiance on the transcript levels of crys in the diencephalon and pituitary gland of honeycomb grouper. After acclimating under a programmable condition (LD = 14:10), fish (3 to 5 individuals per each sampling point) were exposed to artificial full moon and new moon condition and tissues were collected in one-week interval (W1 to W4) at ZT18. The mRNA abundance of crys was measured using qPCR. The data were normalized by determining the amount of β-actin and ef1α. Each point shows comparison of the fold change in the expression levels between the artificial new moon and full moon. Each data is shown as means ± SEM. Different letters indicate statistically significant difference (one-way ANOVA); cry1 (P = 0.10), cry2 (P < 0.05), and cry3 (P < 0.05) for the diencephalon; cry1 (P < 0.05), cry2 (P < 0.05), and cry3 (P = 0.23) for the pituitary gland. Asterisks show statistical difference (P < 0.05) from the new moon condition. The source data and statistical analysis data are included in Additional files 3 and 4, respectively