Fig. 4

Schematic reconstruction of phalloidin stained musculature (a-c) and nervous system of the copulatory organ as revealed by immunoreacrtivity against acetylated α-tubulin (d-f) and serotonin (g-i) of male Dinophilidae, based on CLSM z-stacks. Morphology of male Trilobodrilus axi (a, d, g), Dinophilus vorticoides (b, e, h, including orange pigmentation) and D. gyrociliatus (c, f, i) in ventral (large drawing), and lateral view (small drawing). Larger drawings only showing ventral half of animal (all dorsal muscles and nerves omitted, except for D. gyrociliatus, whose reproductive system extends further dorsally). Musculature of D. gyrociliatus copulatory organ highly simplified, for more details see [18, 21, 22]. Thin black lines in tip of penis cone in D. vorticoides and D. gyrociliatus represent rod-like secretes of stylet glands. Body wall muscles in a-c) are shown more hyaline to allow for a better presentation of muscles of the reproductive system. Abbreviations: am – anchor muscles, avc – anterior ventral ciliation, cb – ciliary band, cm – circular muscle, com5, 6 – fifth/sixth body commissure, cpfm – circumpenial fibre mass, dpc – dorsal penial commissure, gp – gonopore, lm – longitudinal body wall muscle, ln – laternal nerve, lolm – loops of the penis sheath formed by the longitudinal body wall muscles, mvn – medioventral nerve, nsv – nerves of the seminal vesicles, pcm – penis cone muscle, pmn – paramedian nerve, pn – peripheral nerve, ps – penis sheath, pvc – posterior ventral ciliation, sc – sensory cilium, spd – spermioduct, sph – sphincter, sv – seminal vesicle, tcom – terminal commissure, test – testis, vg – ventral ganglion, vllm – ventrolateral longitudinal nerve, vlm – ventral longitudinal muscle(s), vlnc – ventrolateral nerve cord, vpc – ventral penial commissure