Fig. 7

Immunofluorescence staining of whole mount (merged bright field and fluorescence), macerated cell (merged phase contrast and fluorescence), and footprints with anti-AE03 and 3G11 antibodies. a Whole mount, b Macerated basal disc cell, c apical side of macerated cell imaged with super-resolution microscopy, and d footprint stained with AE03 antibody. e Whole mount, f macerated basal disc cell, g apical side of macerated cell imaged with super-resolution microscopy and h footprint stained with 3G11 antibody. Both AE03 and 3G11 have affinity to basal disc cells. Their corresponding antigens are eventually secreted and become a component of the adhesive anchoring Hydra polyps underwater. Scale bars 200 μm (a), 10 μm (b, f), 1 μm (c, g), 15 μm (d, h), 400 μm (e). Abbreviations: bd, basal disc; arrows, indicate bud; arrowheads, indicate nematocysts