Fig. 7

Regeneration of the GABA-like elements of Pygospio elegans nervous system. a Growing head, 3 dpa (lateral view). Yellow arrowheads – GABA-like nervous fibers. b Growing “tail”, 3 dpa (lateral view). c, c’. Growing head, 4 dpa (c – dorsolateral view, c’ - dorsal view). Yellow arrowheads - GABA-like nervous fibers, white arrowheads – first GABA-positive neurons. d Growing “tail”, 4 dpa (ventral view). e Growing head and thoracic segments, 7 dpa (ventral view). Yellow arrows – newly grown thoracic segments. f Growing head, 7 dpa (dorsal view). White arrowheads – GABA-positive neurons. g Growing “tail”, 7 dpa (ventral view). Yellow arrows – newly grown segments. White dashed line marks the cut line. GABA-like elements – green, acetylated α-tubulin-like elements – magenta. Abbreviations: dn – dorsal longitudinal nerve, ln – lateral longitudinal nerve, py – pygidium, vnc – ventral nerve cord