Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits

Table 2 Ranking values of reference genes stability calculated by BestKeeper based on CT in three different muscle tissues of these two species of rabbits

Rank	*Longissimus dorsi* muscle			Abdominal wall muscle			*Quadriceps femoris* muscle
Rank	Gene	Std dev [± CT]	Coeff. of corr.	Gene	Std dev [± CT]	Coeff. of corr.	Gene	Std dev [± CT]	Coeff. of corr.
1	RN18S	0.68	0.625	SDHA	0.20	0.786	RN18S	0.38	0.421
2	HPRT1	0.89	0.979	HPRT1	0.21	0.780	ACTB	0.61	0.889
3	GAPDH	0.90	0.992	GAPDH	0.26	0.257	SDHA	0.68	0.816
4	RPL13A	0.92	0.973	RPL13A	0.27	0.834	HPRT1	0.69	0.992
5	SDHA	1.13	0.895	ACTB	0.36	0.915	PPIC	0.71	0.753
6	ACTB	1.16	0.963	RN18S	0.41	0.001	RPL13A	0.76	0.985
7	PPIC	1.17	0.868	B2M	0.53	0.871	GAPDH	0.77	0.892
8	B2M	1.69	0.907	PPIC	0.73	0.813	B2M	1.23	0.882

ISSN: 2056-3132