Table 1 RT-qPCR primers of H. rugulosus (TT frogs) and H. rugulosus (CT frogs)
Gene | Forward primers (5′–3′) | Reverse primers (5′–3′) |
|---|---|---|
TT-COI | CGGCTTCGGAATCATCTC | TTAGGTCGGTCGTGAATATG |
CT-COI | CTCCTTGGTGGGGGTTAT | GGCTTCTTCAAATGTGTGG |
TT-COII1(TT- COII2) | CAACCGCATAGTAACT(C)CC | GGTGAGCAGTAATAAAAGCAG |
CT-COII | CTGCTTTTATTACTGCCCA | TTAGTCAGGAGAGGAAGTTG |
TT-COIII | GACGAGATGTTGTCCGAG | ATTGTAGAATGCCCAGAAG |
CT-COIII | CTCCACAGTCCTTGTATTTG | AGAGGATTATGCCAAAGC |
TT-ATP8 | TGGTACATGGTCCTTCTCT | GTTCAGTGTCAGATGTTAGTC |
CT-ATP81(CT-ATP82) | CCCTGGTACTCG(C)ATTCTTC | CGCTGCTTTGAGAAGTG(A)G |
TT-ATP6 | CTGTCATCCTTAGTGGTCTT | ATGAGAACGGTGGCTAAC |
CT-ATP61(CT-ATP62) | ACTTTC(T)TACCAGAGGGCAC | AGGTGGATAAGCAGGTGAC |
TT-ND1 | GCTCTAATGTGATTCGTATCC | TTCGGCAAGGAAGAATAGG |
CT-ND1 | CAACTCCAAATACGCTCTC | TGGGCGTAGGTGAAGTTA |
TT-ND2 | TGACTACTCGCTTGAATCG | GTTGCTGAGAATAGGATAAGTG |
CT-ND2 | ATTCTCAGCGACCATAAAC | TTACTTCGGGCATTCAGA |
TT-ND3 | GTGCTCATATTCCTATCAGTC | CCGCATTCGTATGGAGATA |
CT-ND3 | CTACCCTTCTGGTCATCG | CAGGAAAAATCGTATTGAGTA |
TT-ND4 | AACAATCATCACCGTCCAA | GTCAGCAGGTTAGCAGAAT |
CT-ND41(CT-ND42) | AAGGCTTCTTTATT(C)GCTGA | GGAA(G)TAGCCAGCAGGTTA |
TT-ND4L | GGCATGATACTTACCATCTTC | GCTGTGGCAATCATTAAGG |
CT-ND4L1(CT-ND4L2) | TCAACTGAAC(T)TCAACCACTC | AGAAGATTTAGCGTTTTGAG |
TT-ND5 | TACCTGTCCACTCCTTCG | TCTGGTAGTGTCGCTTCT |
CT-ND5 | TTTACAGTGTTTGCGGTC | ATAAGGACGAACAGTGGG |
TT-ND5-1 | AAGACACCCAAGACATTCG | TCCTTAGAGTAGAATGCGATG |
TT-ND5-2 | CCTCCTCCTCACTCTTGC | GTTTTTACGACTTGTGGTTC |
TT-ND6 | AATATAAGCCGCTAGGTAGAC | GGGTATAATGGTGGTATTTGC |
CT-ND6 | CCAACCCGTCAAACAACA | TTGCTTATAGTGCTGCTCTT |
TT-Cytb | GGCGTTGTCCTTCTCTTC | AGGTTGGTGATGACTGTTG |
CT-Cytb | GCTTTCGTGGGCTATGTC | AGTTAGCGTGGCGTTGTC |
Actin | GATCTGGCATCACACTTTCT | TGGGTGACACCATCACCAGA |