Table 1 Results of two-way Analysis of Variance (ANOVA) and the Tukey HSD test for the effects of Maturity stage (4A and 4B), Oocyte development stage (Mature / Immature), and the interactions of the two terms

Df

Sum Sq

F value

Pr(>F)

 Maturity stage

1

976.2

76.342

<  0.001^*

 Oocyte stage

1

136

10.637

<  0.01^*

 Maturity stage * Oocyte stage

1

72.2

5.645

<  0.05^*

 Residuals

48

613.8

diff

lwr

upr

p adj

 4B - 4A

−8.906

−10.955

−6.857

<  0.001^*

 Mature – Immature

−3.235

−5.230

−1.241

<  0.01^*

 4B Immature - 4A Immature

−6.484

−10.321

−2.648

<  0.001^*

 4A Mature - 4A Immature

−1.372

−4.736

1.993

0.7

 4B Mature - 4A Immature

−12.699

−16.536

−8.863

<  0.001^*

 4A Mature- 4B Immature

5.112

1.276

8.949

<  0.01^*

 4B Mature - 4B Immature

−6.215

−10.471

− 1.959

<  0.01^*

 4B Mature- 4A Mature

−11.328

−15.164

−7.491

<  0.001^*