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Fig. 4 | BMC Zoology

Fig. 4

From: Investigation of epididymal proteins and general sperm membrane characteristics of Formosan pangolin (Manis pentadactyla pentadactyla)

Fig. 4

Transmission electron micrographs demonstrated multi-layer membrane structural at pangolin sperm head. a We observed multiple membrane layers presented at the pangolin sperm head region; cross section of sperm head region (inset image) further confirmed this observation. b Acrosome swelling was observed likely representing acrosome reacting process as reported in other species. Cross section of sperm head showed an expansion of sperm acrosome. c Membrane fusion event was detected at the sperm head; fusion vesicles (marked with asterisks) can be detected underneath intact plasma membrane. The spermatozoa on the right likely represent acrosome reacted sperm with release plasma membrane and fusion vesicles. AR: acrosome, N: nucleus; PM: plasma membrane; NM: nuclear membrane; yellow dashed line illustrated the cross-section image observed in the inset figures

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