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Fig. 2 | BMC Zoology

Fig. 2

From: Moonlight is a key entrainer of lunar clock in the brain of the tropical grouper with full moon preference

Fig. 2

Daily changes in the transcript levels of crys in the diencephalon and pituitary gland of honeycomb grouper. Fish (3 to 5 individuals per each sampling point) were reared under a programmable photoperiod (LD = 14:10) and tissues were taken from fish at 4 h intervals. The mRNA abundance of crys was measured using qPCR. The data were normalized by determining the amount of β-actin and ef1α. Each data is shown as means ± SEM. Different letters indicate statistically significant difference (ANOVA and Kruskal-Wallis Test*); cry1 (P < 0.01), cry2 (P < 0.01), and cry3 (P = 0.58) for the diencephalon; cry1 (P < 0.01*), cry2 (P < 0.01), and cry3 (P = 0.06) for the pituitary gland. The source data and statistical analysis data are included in Additional files 3 and 4, respectively

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