Fig. 2From: Moonlight is a key entrainer of lunar clock in the brain of the tropical grouper with full moon preferenceDaily changes in the transcript levels of crys in the diencephalon and pituitary gland of honeycomb grouper. Fish (3 to 5 individuals per each sampling point) were reared under a programmable photoperiod (LD = 14:10) and tissues were taken from fish at 4 h intervals. The mRNA abundance of crys was measured using qPCR. The data were normalized by determining the amount of β-actin and ef1α. Each data is shown as means ± SEM. Different letters indicate statistically significant difference (ANOVA and Kruskal-Wallis Test*); cry1 (P < 0.01), cry2 (P < 0.01), and cry3 (P = 0.58) for the diencephalon; cry1 (P < 0.01*), cry2 (P < 0.01), and cry3 (P = 0.06) for the pituitary gland. The source data and statistical analysis data are included in Additional files 3 and 4, respectivelyBack to article page