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Fig. 3 | BMC Zoology

Fig. 3

From: Sea anemones (Exaiptasia pallida) use a secreted adhesive and complex pedal disc morphology for surface attachment

Fig. 3

Histological staining of the pedal disc (i) and corresponding footprints (ii). (A) PAS and haematoxylin staining. Pink = general polysaccharides, blue = nuclei. Absence of spirocysts within the basal tissue compared to Fig. 1. (B) Footprint strongly stained for protein using Coomasie blue. (C) PNA labelling (red) and nuclei (DAPI: blue). (D) WGA labelling. (Di) Fluorescent and bright field images overlapped. (E) Muco-polysaccharide staining using Alcian blue pH 2.5 (D,E) black arrow indicates cells stained strongly by WGA and Alcian blue pH 2.5. White arrow indicates cell stained strongly with Alcian blue and weakly by WGA. (F) Highly acidic muco-polysaccharides stained using Alcian blue pH 1. (G) β-glycan staining using calcofluor white (Gi is a fluorescent 3D deconvoluted image). (H) Quinone staining using Nitro-Blue Tetrazolium. Scale bars = 20 μm

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