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Fig. 5 | BMC Zoology

Fig. 5

From: Drosophila melanogaster chemical ecology revisited: 2-D distribution maps of sex pheromones on whole virgin and mated flies by mass spectrometry imaging

Fig. 5

The transfer of male sex pheromones cVA and CH503 within one pair of intact six-day-old copulated D. melanogaster flies. The dataset was obtained by LDI-TOF MSI in positive ion mode. (a) A 2-D ion intensity map of cVA at m/z 349.5 ([M + K]+) with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ±0.2 Da. (b) The observed mass shift of cVA represented as a 2-D ion intensity map in PlusMinus 1 color scale. (c) The mass shift correction of m/z 349.5 signal made after applying the recalibration method. d) A 2-D ion intensity map of CH503 at m/z 503.8 ([M + K]+) with signal intensity in voxel view and rainbow-color coded within a mass window of ±0.3 Da. (e) The observed mass shift of CH503 represented as a 2-D ion intensity map in PlusMinus 1 color scale. (f) The mass shift correction of m/z 503.8 signal made after applying the recalibration method

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